Composite
Part:BBa_K4167000:Design
Designed by: Xinzichu Xie Group: iGEM22_ICJFLS (2022-10-01)
toehold switch-amilCP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pET-28a-toehold switch-amilCP plasmid was designed to comprise some antisense sequence of miRNA 34a-5p, constant sequence, and part sequence of miRNA 34a-5p, which form a toehold switch, as well as the gene of marker protein amilCP. At the presence of miRNA 34a-5p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of amilCP, which is easily measured.
Source
miRNA 34a-5p comes from human, amilCP comes from Acropora millepora.